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Difco r2a medium difco
R2a Medium Difco, supplied by Difco, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r2a+medium+difco/r2a+medium+difco/pm38656427-29-16-18
Average 90 stars, based on 1 article reviews
r2a medium difco - by Bioz Stars, 2026-09
90/100 stars

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Suspension:

Article Title: Hymenobacter puniceus sp. nov., radiation resistant bacteria isolated from soil in South Korea.
Article Snippet: Two novel Gram-negative bacterial strains BT190 and BT191 were isolated from soil collected in Uijeongbu city (37 4405500N, 127 0202000E), Korea.. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT190 and BT191 belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia).. The level of 16S rRNA gene sequence similarity between the strains BT190 and BT190 was 99.5%.

Article Title: Genomic Insights Into New Species of the Genus Halomicroarcula Reveals Potential for New Osmoadaptative Strategies in Halophilic Archaea
Article Snippet: Strains F24A T , F28, F27 T , and F13 T were isolated and routinely grown in R2A medium (Difco) (pH adjusted to 7.5) supplemented with 25% (w/v) seawater salt solution prepared by dilution of SW 30% (w/v) stock solution containing (g/L): NaCl, 195; MgCl 2 ⋅6H 2 O, 32.5; MgSO 4 ⋅7H 2 O, 50.8; CaCl 2 , 0.83; KCl, 5.0; NaHCO 3 , 0.17; NaBr, 0.58.

Article Title: Hymenobacter negativus sp. nov., bacteria isolated from mountain soil collected in South Korea.
Article Snippet: Two novel Gram-negative bacterial strains BT442 and BT584 were isolated from dry soil collected in mountains Busan and Guri, Korea during wintertime.. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT442 and BT584 both belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia).. Strain BT442 was closely related to Hymenobacter soli PB17 (98.0% 16S rRNA gene similarity) and Hymenobacter terrae POA9 (97.6%).

Article Title: Cohnella candidum sp. nov., radiation-resistant bacterium from soil.
Article Snippet: A Gram-stain positive, endospore-forming, circular, convex, cream colored, designated strain 18JY8-7, was isolated from soil collected in Jeju Island, South Korea.. Phylogenetic analysis using 16S rRNA gene sequences showed that strain 18JY8-7 formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and is closely related to Cohnella rhizosphaerae (96.1%, sequence similarity) and Cohnella xylanilytica (96.0%).. Optimal growth occurred at 30 C, pH 6.5 and in the absence of NaCl.

Article Title: Freezing and thawing in Antarctica: characterization of antifreeze protein (AFP) producing microorganisms isolated from King George Island, Antarctica.
Article Snippet: Although freeze-thaw cycles limit the survival of most organisms, several microorganisms can survive in the Antarctic extreme environments due to their resistance to freezing [1].. These microorganisms present a series of adaptations to cold, including increased membrane fluidity and more efficient subzero metabolism, as well as the production of cryoprotective substances, such as exopolysaccharides [2, 3] and antifreeze proteins (AFP) [4, 5].. Previous studies have reported the freezing resistance of microorganisms [6–10], with some producing AFPs that effectively inhibit ice formation.

Article Title: The effect of plant growth-promoting rhizobacteria on the phytoextraction of Cd and Zn by Brassica napus L.
Article Snippet: The test strains Bacteroidetes bacterium (Ba), Pseudomonas fluorescens (Pf) and Variovorax sp. (Va) were selected in advance for their in vitro capability for growth promotion of rapeseed in the presence of increased concentrations of Cd, Cu, Pb and Zn in the medium.. In the pot experiment the strains were used for single Ba, Pf, Va or combined Ba+Pf, Ba+Va, Pf+Va, Ba+Pf+Va inoculation of B. napus growing in contaminated soil from alluvial deposits.. The positive effect of bacterial strains on plant growth was observed in vitro, but was not confirmed in situ in the contaminated soil, where the tested strains inhibited biomass production, rather than stimulating it.

Article Title: Paenibacillus albus sp. nov., a UV radiation-resistant bacterium isolated from soil in Korea.
Article Snippet: A novel Gram-stain-positive, motile, white color and endospore-forming bacterium, designated 18JY67-1T, was isolated from soil in Jeju Island, Korea.. The strain grow at 15–42 °C (optimum 30 °C) in R2A medium at pH (6.0–9.5) (optimum 7.5).. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 18JY67-1T formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and was closely related to Paenibacillus rhizoryzae (KP675984; 96.9% 16S rRNA gene sequence similarity).

Starch:

Article Title: Hymenobacter puniceus sp. nov., radiation resistant bacteria isolated from soil in South Korea.
Article Snippet: Two novel Gram-negative bacterial strains BT190 and BT191 were isolated from soil collected in Uijeongbu city (37 4405500N, 127 0202000E), Korea.. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT190 and BT191 belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia).. The level of 16S rRNA gene sequence similarity between the strains BT190 and BT190 was 99.5%.

Article Title: Genomic Insights Into New Species of the Genus Halomicroarcula Reveals Potential for New Osmoadaptative Strategies in Halophilic Archaea
Article Snippet: Strains F24A T , F28, F27 T , and F13 T were isolated and routinely grown in R2A medium (Difco) (pH adjusted to 7.5) supplemented with 25% (w/v) seawater salt solution prepared by dilution of SW 30% (w/v) stock solution containing (g/L): NaCl, 195; MgCl 2 ⋅6H 2 O, 32.5; MgSO 4 ⋅7H 2 O, 50.8; CaCl 2 , 0.83; KCl, 5.0; NaHCO 3 , 0.17; NaBr, 0.58.

Article Title: Hymenobacter negativus sp. nov., bacteria isolated from mountain soil collected in South Korea.
Article Snippet: Two novel Gram-negative bacterial strains BT442 and BT584 were isolated from dry soil collected in mountains Busan and Guri, Korea during wintertime.. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT442 and BT584 both belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia).. Strain BT442 was closely related to Hymenobacter soli PB17 (98.0% 16S rRNA gene similarity) and Hymenobacter terrae POA9 (97.6%).

Article Title: Cohnella candidum sp. nov., radiation-resistant bacterium from soil.
Article Snippet: A Gram-stain positive, endospore-forming, circular, convex, cream colored, designated strain 18JY8-7, was isolated from soil collected in Jeju Island, South Korea.. Phylogenetic analysis using 16S rRNA gene sequences showed that strain 18JY8-7 formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and is closely related to Cohnella rhizosphaerae (96.1%, sequence similarity) and Cohnella xylanilytica (96.0%).. Optimal growth occurred at 30 C, pH 6.5 and in the absence of NaCl.

Article Title: Freezing and thawing in Antarctica: characterization of antifreeze protein (AFP) producing microorganisms isolated from King George Island, Antarctica.
Article Snippet: Although freeze-thaw cycles limit the survival of most organisms, several microorganisms can survive in the Antarctic extreme environments due to their resistance to freezing [1].. These microorganisms present a series of adaptations to cold, including increased membrane fluidity and more efficient subzero metabolism, as well as the production of cryoprotective substances, such as exopolysaccharides [2, 3] and antifreeze proteins (AFP) [4, 5].. Previous studies have reported the freezing resistance of microorganisms [6–10], with some producing AFPs that effectively inhibit ice formation.

Article Title: The effect of plant growth-promoting rhizobacteria on the phytoextraction of Cd and Zn by Brassica napus L.
Article Snippet: The test strains Bacteroidetes bacterium (Ba), Pseudomonas fluorescens (Pf) and Variovorax sp. (Va) were selected in advance for their in vitro capability for growth promotion of rapeseed in the presence of increased concentrations of Cd, Cu, Pb and Zn in the medium.. In the pot experiment the strains were used for single Ba, Pf, Va or combined Ba+Pf, Ba+Va, Pf+Va, Ba+Pf+Va inoculation of B. napus growing in contaminated soil from alluvial deposits.. The positive effect of bacterial strains on plant growth was observed in vitro, but was not confirmed in situ in the contaminated soil, where the tested strains inhibited biomass production, rather than stimulating it.

Article Title: Paenibacillus albus sp. nov., a UV radiation-resistant bacterium isolated from soil in Korea.
Article Snippet: A novel Gram-stain-positive, motile, white color and endospore-forming bacterium, designated 18JY67-1T, was isolated from soil in Jeju Island, Korea.. The strain grow at 15–42 °C (optimum 30 °C) in R2A medium at pH (6.0–9.5) (optimum 7.5).. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 18JY67-1T formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and was closely related to Paenibacillus rhizoryzae (KP675984; 96.9% 16S rRNA gene sequence similarity).

Isolation:

Article Title: Hymenobacter puniceus sp. nov., radiation resistant bacteria isolated from soil in South Korea.
Article Snippet: Two novel Gram-negative bacterial strains BT190 and BT191 were isolated from soil collected in Uijeongbu city (37 4405500N, 127 0202000E), Korea.. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT190 and BT191 belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia).. The level of 16S rRNA gene sequence similarity between the strains BT190 and BT190 was 99.5%.

Article Title: Genomic Insights Into New Species of the Genus Halomicroarcula Reveals Potential for New Osmoadaptative Strategies in Halophilic Archaea
Article Snippet: Strains F24A T , F28, F27 T , and F13 T were isolated and routinely grown in R2A medium (Difco) (pH adjusted to 7.5) supplemented with 25% (w/v) seawater salt solution prepared by dilution of SW 30% (w/v) stock solution containing (g/L): NaCl, 195; MgCl 2 ⋅6H 2 O, 32.5; MgSO 4 ⋅7H 2 O, 50.8; CaCl 2 , 0.83; KCl, 5.0; NaHCO 3 , 0.17; NaBr, 0.58.

Article Title: Hymenobacter negativus sp. nov., bacteria isolated from mountain soil collected in South Korea.
Article Snippet: Two novel Gram-negative bacterial strains BT442 and BT584 were isolated from dry soil collected in mountains Busan and Guri, Korea during wintertime.. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT442 and BT584 both belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia).. Strain BT442 was closely related to Hymenobacter soli PB17 (98.0% 16S rRNA gene similarity) and Hymenobacter terrae POA9 (97.6%).

Article Title: Cohnella candidum sp. nov., radiation-resistant bacterium from soil.
Article Snippet: A Gram-stain positive, endospore-forming, circular, convex, cream colored, designated strain 18JY8-7, was isolated from soil collected in Jeju Island, South Korea.. Phylogenetic analysis using 16S rRNA gene sequences showed that strain 18JY8-7 formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and is closely related to Cohnella rhizosphaerae (96.1%, sequence similarity) and Cohnella xylanilytica (96.0%).. Optimal growth occurred at 30 C, pH 6.5 and in the absence of NaCl.

Article Title: Freezing and thawing in Antarctica: characterization of antifreeze protein (AFP) producing microorganisms isolated from King George Island, Antarctica.
Article Snippet: Although freeze-thaw cycles limit the survival of most organisms, several microorganisms can survive in the Antarctic extreme environments due to their resistance to freezing [1].. These microorganisms present a series of adaptations to cold, including increased membrane fluidity and more efficient subzero metabolism, as well as the production of cryoprotective substances, such as exopolysaccharides [2, 3] and antifreeze proteins (AFP) [4, 5].. Previous studies have reported the freezing resistance of microorganisms [6–10], with some producing AFPs that effectively inhibit ice formation.

Article Title: The effect of plant growth-promoting rhizobacteria on the phytoextraction of Cd and Zn by Brassica napus L.
Article Snippet: The test strains Bacteroidetes bacterium (Ba), Pseudomonas fluorescens (Pf) and Variovorax sp. (Va) were selected in advance for their in vitro capability for growth promotion of rapeseed in the presence of increased concentrations of Cd, Cu, Pb and Zn in the medium.. In the pot experiment the strains were used for single Ba, Pf, Va or combined Ba+Pf, Ba+Va, Pf+Va, Ba+Pf+Va inoculation of B. napus growing in contaminated soil from alluvial deposits.. The positive effect of bacterial strains on plant growth was observed in vitro, but was not confirmed in situ in the contaminated soil, where the tested strains inhibited biomass production, rather than stimulating it.

Article Title: Paenibacillus albus sp. nov., a UV radiation-resistant bacterium isolated from soil in Korea.
Article Snippet: A novel Gram-stain-positive, motile, white color and endospore-forming bacterium, designated 18JY67-1T, was isolated from soil in Jeju Island, Korea.. The strain grow at 15–42 °C (optimum 30 °C) in R2A medium at pH (6.0–9.5) (optimum 7.5).. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 18JY67-1T formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and was closely related to Paenibacillus rhizoryzae (KP675984; 96.9% 16S rRNA gene sequence similarity).

Incubation:

Article Title: Hymenobacter puniceus sp. nov., radiation resistant bacteria isolated from soil in South Korea.
Article Snippet: Two novel Gram-negative bacterial strains BT190 and BT191 were isolated from soil collected in Uijeongbu city (37 4405500N, 127 0202000E), Korea.. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT190 and BT191 belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia).. The level of 16S rRNA gene sequence similarity between the strains BT190 and BT190 was 99.5%.

Article Title: Genomic Insights Into New Species of the Genus Halomicroarcula Reveals Potential for New Osmoadaptative Strategies in Halophilic Archaea
Article Snippet: Strains F24A T , F28, F27 T , and F13 T were isolated and routinely grown in R2A medium (Difco) (pH adjusted to 7.5) supplemented with 25% (w/v) seawater salt solution prepared by dilution of SW 30% (w/v) stock solution containing (g/L): NaCl, 195; MgCl 2 ⋅6H 2 O, 32.5; MgSO 4 ⋅7H 2 O, 50.8; CaCl 2 , 0.83; KCl, 5.0; NaHCO 3 , 0.17; NaBr, 0.58.

Article Title: Hymenobacter negativus sp. nov., bacteria isolated from mountain soil collected in South Korea.
Article Snippet: Two novel Gram-negative bacterial strains BT442 and BT584 were isolated from dry soil collected in mountains Busan and Guri, Korea during wintertime.. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT442 and BT584 both belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia).. Strain BT442 was closely related to Hymenobacter soli PB17 (98.0% 16S rRNA gene similarity) and Hymenobacter terrae POA9 (97.6%).

Article Title: Cohnella candidum sp. nov., radiation-resistant bacterium from soil.
Article Snippet: A Gram-stain positive, endospore-forming, circular, convex, cream colored, designated strain 18JY8-7, was isolated from soil collected in Jeju Island, South Korea.. Phylogenetic analysis using 16S rRNA gene sequences showed that strain 18JY8-7 formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and is closely related to Cohnella rhizosphaerae (96.1%, sequence similarity) and Cohnella xylanilytica (96.0%).. Optimal growth occurred at 30 C, pH 6.5 and in the absence of NaCl.

Article Title: Freezing and thawing in Antarctica: characterization of antifreeze protein (AFP) producing microorganisms isolated from King George Island, Antarctica.
Article Snippet: Although freeze-thaw cycles limit the survival of most organisms, several microorganisms can survive in the Antarctic extreme environments due to their resistance to freezing [1].. These microorganisms present a series of adaptations to cold, including increased membrane fluidity and more efficient subzero metabolism, as well as the production of cryoprotective substances, such as exopolysaccharides [2, 3] and antifreeze proteins (AFP) [4, 5].. Previous studies have reported the freezing resistance of microorganisms [6–10], with some producing AFPs that effectively inhibit ice formation.

Article Title: The effect of plant growth-promoting rhizobacteria on the phytoextraction of Cd and Zn by Brassica napus L.
Article Snippet: The test strains Bacteroidetes bacterium (Ba), Pseudomonas fluorescens (Pf) and Variovorax sp. (Va) were selected in advance for their in vitro capability for growth promotion of rapeseed in the presence of increased concentrations of Cd, Cu, Pb and Zn in the medium.. In the pot experiment the strains were used for single Ba, Pf, Va or combined Ba+Pf, Ba+Va, Pf+Va, Ba+Pf+Va inoculation of B. napus growing in contaminated soil from alluvial deposits.. The positive effect of bacterial strains on plant growth was observed in vitro, but was not confirmed in situ in the contaminated soil, where the tested strains inhibited biomass production, rather than stimulating it.

Article Title: Paenibacillus albus sp. nov., a UV radiation-resistant bacterium isolated from soil in Korea.
Article Snippet: A novel Gram-stain-positive, motile, white color and endospore-forming bacterium, designated 18JY67-1T, was isolated from soil in Jeju Island, Korea.. The strain grow at 15–42 °C (optimum 30 °C) in R2A medium at pH (6.0–9.5) (optimum 7.5).. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 18JY67-1T formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and was closely related to Paenibacillus rhizoryzae (KP675984; 96.9% 16S rRNA gene sequence similarity).

Purification:

Article Title: Hymenobacter puniceus sp. nov., radiation resistant bacteria isolated from soil in South Korea.
Article Snippet: Two novel Gram-negative bacterial strains BT190 and BT191 were isolated from soil collected in Uijeongbu city (37 4405500N, 127 0202000E), Korea.. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT190 and BT191 belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia).. The level of 16S rRNA gene sequence similarity between the strains BT190 and BT190 was 99.5%.

Article Title: Genomic Insights Into New Species of the Genus Halomicroarcula Reveals Potential for New Osmoadaptative Strategies in Halophilic Archaea
Article Snippet: Strains F24A T , F28, F27 T , and F13 T were isolated and routinely grown in R2A medium (Difco) (pH adjusted to 7.5) supplemented with 25% (w/v) seawater salt solution prepared by dilution of SW 30% (w/v) stock solution containing (g/L): NaCl, 195; MgCl 2 ⋅6H 2 O, 32.5; MgSO 4 ⋅7H 2 O, 50.8; CaCl 2 , 0.83; KCl, 5.0; NaHCO 3 , 0.17; NaBr, 0.58.

Article Title: Hymenobacter negativus sp. nov., bacteria isolated from mountain soil collected in South Korea.
Article Snippet: Two novel Gram-negative bacterial strains BT442 and BT584 were isolated from dry soil collected in mountains Busan and Guri, Korea during wintertime.. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT442 and BT584 both belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia).. Strain BT442 was closely related to Hymenobacter soli PB17 (98.0% 16S rRNA gene similarity) and Hymenobacter terrae POA9 (97.6%).

Article Title: Cohnella candidum sp. nov., radiation-resistant bacterium from soil.
Article Snippet: A Gram-stain positive, endospore-forming, circular, convex, cream colored, designated strain 18JY8-7, was isolated from soil collected in Jeju Island, South Korea.. Phylogenetic analysis using 16S rRNA gene sequences showed that strain 18JY8-7 formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and is closely related to Cohnella rhizosphaerae (96.1%, sequence similarity) and Cohnella xylanilytica (96.0%).. Optimal growth occurred at 30 C, pH 6.5 and in the absence of NaCl.

Article Title: Freezing and thawing in Antarctica: characterization of antifreeze protein (AFP) producing microorganisms isolated from King George Island, Antarctica.
Article Snippet: Although freeze-thaw cycles limit the survival of most organisms, several microorganisms can survive in the Antarctic extreme environments due to their resistance to freezing [1].. These microorganisms present a series of adaptations to cold, including increased membrane fluidity and more efficient subzero metabolism, as well as the production of cryoprotective substances, such as exopolysaccharides [2, 3] and antifreeze proteins (AFP) [4, 5].. Previous studies have reported the freezing resistance of microorganisms [6–10], with some producing AFPs that effectively inhibit ice formation.

Article Title: The effect of plant growth-promoting rhizobacteria on the phytoextraction of Cd and Zn by Brassica napus L.
Article Snippet: The test strains Bacteroidetes bacterium (Ba), Pseudomonas fluorescens (Pf) and Variovorax sp. (Va) were selected in advance for their in vitro capability for growth promotion of rapeseed in the presence of increased concentrations of Cd, Cu, Pb and Zn in the medium.. In the pot experiment the strains were used for single Ba, Pf, Va or combined Ba+Pf, Ba+Va, Pf+Va, Ba+Pf+Va inoculation of B. napus growing in contaminated soil from alluvial deposits.. The positive effect of bacterial strains on plant growth was observed in vitro, but was not confirmed in situ in the contaminated soil, where the tested strains inhibited biomass production, rather than stimulating it.

Article Title: Paenibacillus albus sp. nov., a UV radiation-resistant bacterium isolated from soil in Korea.
Article Snippet: A novel Gram-stain-positive, motile, white color and endospore-forming bacterium, designated 18JY67-1T, was isolated from soil in Jeju Island, Korea.. The strain grow at 15–42 °C (optimum 30 °C) in R2A medium at pH (6.0–9.5) (optimum 7.5).. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 18JY67-1T formed a distinct lineage within the family Paenibacillaceae (order Bacillales, class Bacilli), and was closely related to Paenibacillus rhizoryzae (KP675984; 96.9% 16S rRNA gene sequence similarity).



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